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Home >> Antibodies >> Daxx Antibody

Daxx Antibody (R30907)

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  Catalog No Formulation Size Price (USD)  
Image R30907 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human tonsil with Daxx antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with Daxx antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Daxx antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Daxx antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) HepG2, 3) A549, 4) Raji, 5) rat testis and 6) mouse testis lysate with Daxx antibody. Predicted molecular weight ~81 kDa but routinely observed at ~120 kDa, the slow SDS-PAGE migration possibly due to the proteins high acidic residue content.
Flow cytometry testing of human Raji cells with Daxx antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Daxx antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9UER7
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Daxx antibody is available for research use only.
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Description

Death-domain associated protein, also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. The gene encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses byYang et al.(1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of the protein interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Daxx antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 325-341 (QERRHLDLIYNFGCHLT) were used as the immunogen for this Daxx antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Daxx antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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