- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 8/18 Antibody [clone 5D3] (V3316)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3316-0.5ML | Bioreactor concentrate with 0.05% sodium azide | 0.5 ml | 519 | |
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V3316-0.1ML | Bioreactor concentrate with 0.05% sodium azide | 0.1 ml | 229 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Bioreactor concentrate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 5D3 |
| UniProt | P05787 , P05783 |
| Localization | Cytoplasmic |
| Applications | Flow cytometry : 2-5ul/10^6 cells Immunofluorescence : 1:100-1:200 Immunohistochemistry (FFPE) : 1:50-1:100 for 30 min at RT |
| Limitations | This Cytokeratin 8/18 antibody is available for research use only. |
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Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
A cytoskeleton preparation from human breast cancer MCF-7 cells was used as the immunogen for the Cytokeratin 8/18 antibody.
Store the Cytokeratin 8/18 antibody at 2-8oC.
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