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Home >> Antibodies >> Cytokeratin 7 Antibody

Cytokeratin 7 Antibody [clone OV-TL12/30] (V2654)

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  Catalog No Formulation Size Price (USD)  
Image V2654-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2654-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2654SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2654IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone OV-TL12/30); Red=isotype control, Blue= recombinant Cytokeratin 7 antibody.
Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone OV-TL12/30).
Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name OV-TL12/30
Purity Protein G affinity chromatography
UniProt P08729
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Cytokeratin 7 antibody is available for research use only.
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Description

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Application Notes

The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. View the recombinant version of this Cytokeratin 7 antibody.

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.

Storage

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View the recombinant version of this Cytokeratin 7 antibody

   2.   View all Cytokeratin 7 antibodies

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