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Home >> Antibodies >> Cytokeratin 15 Antibody / KRT15

Cytokeratin 15 Antibody / KRT15 [clone KRT15/8312R] (V4501)

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  Catalog No Formulation Size Price (USD)  
Image V4501-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4501-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4501SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human skin tissue with Cytokeratin 15 antibody (clone KRT15/8312R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skin tissue with Cytokeratin 15 antibody (clone KRT15/8312R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 15 antibody (clone KRT15/8312R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT15/8312R
Purity Protein A affinity
UniProt P19012
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Cytokeratin 15 antibody is available for research use only.
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Description

Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. KRT15 is downregulated in activated keratinocytes. It is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Application Notes

Optimal dilution of the Cytokeratin 15 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length KRT15 protein was used as the immunogen for the Cytokeratin 15 antibody.

Storage

Aliquot the Cytokeratin 15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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