• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Cytokeratin 12 Antibody / KRT12

Cytokeratin 12 Antibody / KRT12 (F54280)

  Catalog No Formulation Size Price (USD)  
Image F54280-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54280-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
IHC testing of FFPE human colon carcinoma tissue with Cytokeratin 12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Y79 cell lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of mouse eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of rat eyeball lysate with Cytokeratin 12 antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with Cytokeratin 12 antibody; Blue=isotype control, Green= Cytokeratin 12 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q99456
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This Cytokeratin 12 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Cytokeratin 12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 442-471 from the human protein were used as the immunogen for the Cytokeratin 12 antibody.

Storage

Aliquot the Cytokeratin 12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.