CUX1 Antibody / Homeobox protein cut-like 1 (F49389)

Fluorescent confocal image of human HeLa cell stained with CUX1 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.

Fluorescent staining of human MCF-7 cells with CUX1 antibody. Cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). Green = CUX1 antibody, Red = Phalloidin stained actin.

IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human kidney with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lymph node with CUX1 antibody at 1:25. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Western blot testing of human 1) MCF-7, 2) MOLT-4 and 3) SH-SY5Y cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.

Western blot testing of human 1) HEK293 and 2) K562 cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.

Western blot testing of human MCF7 cell lysate with CUX1 antibody at 1:2000. Expected molecular weight ~200 kDa with smaller isoforms.