CRYAB Antibody | Protein Microarray Validated

	Catalog No	Formulation	Size	Price (USD)
	V3972-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V3972-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V3972SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V3972IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC staining of FFPE human breast tissue with CRYAB antibody (clone CPTC-CRYAB-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE rat heart tissue with CRYAB antibody (clone CPTC-CRYAB-1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Western blot testing of human HeLa lysate with CRYAB antibody (clone CPTC-CRYAB-1). Predicted molecular weight: ~20 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CRYAB antibody (clone CPTC-CRYAB-1). These results demonstrate the foremost specificity of the CPTC-CRYAB-1 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Availability	1-3 business days
Species Reactivity	Human, Rat
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2c, kappa
Clone Name	CPTC-CRYAB-1
Purity	Protein G affinity chromatography
UniProt	P02511
Localization	Cytoplasmic, nuclear
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations	This CRYAB antibody is available for research use only.
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Description

Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta and gamma families, and the beta- and gamma-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha-crystallins consist of three gene products, alpha-A-, alpha-B- and alpha-C-crystallin, which are members of the small heat shock protein family (HSP 20). Alpha-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, alpha-crystallins do not renature these proteins. Expression of alpha-A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human alpha-B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of alpha-B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Application Notes

Optimal dilution of the CRYAB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.