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Home >> Antibodies >> CREB Antibody

CREB Antibody (F41641)

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  Catalog No Formulation Size Price (USD)  
Image F41641-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F41641-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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CREB antibody western blot analysis in HT29 lysate. Predicted molecular weight is 37 kDa but routinely observed at ~43 kDa.
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CREB antibody western blot analysis in HT29 lysate. Predicted molecular weight is 37 kDa but routinely observed at ~43 kDa.
Fluorescent image of A549 cell stained with CREB antibody. Alexa Fluor 488 (green) secondary was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin. Immunoreactivity is localized to the nucleus.
CREB antibody flow cytometric analysis of A549 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P16220
Applications Immunofluorescence : 1:10-1:50
Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This CREB antibody is available for research use only.
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Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Application Notes

Titration of the CREB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 105-132 from the human protein was used as the immunogen for this CREB antibody.

Storage

Aliquot the CREB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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