• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> CPI-17 Antibody / PPP1R14A

CPI-17 Antibody / PPP1R14A (R32782)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R32782 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
IHC testing of FFPE human lung cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
›
IHC testing of FFPE human lung cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human placental tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse testis tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lung tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) rat brain, 2) mouse brain and 3) human PANC1 lysate with CPI-17 antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
Flow cytometry testing of human U-2 OS cells with CPI-17 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CPI-17 antibody.
IHC testing of FFPE human U-2 OS cells with CPI-17 antibody (green) and DAPI (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt Q96A00
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/10^6 cells
Immunofluorescence : 2-4ug/ml
Limitations This CPI-17 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Application Notes

Optimal dilution of the CPI-17 antibody should be determined by the researcher.

Immunogen

Amino acids L30-Q126 from human CPI-17 alpha were used as the immunogen for the CPI-17 antibody.

Storage

After reconstitution, the CPI-17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all CPI-17 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.