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Home >> Antibodies >> Connexin 32 Antibody / GJB1

Connexin 32 Antibody / GJB1 [clone M12.13] (V3306)

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  Catalog No Formulation Size Price (USD)  
Image V3306-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3306-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3306SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free Connexin 32 Antibody (clone M12.13). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free Connexin 32 Antibody (clone M12.13). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name M12.13
Purity Protein G affinity chromatography
UniProt P08034
Localization Cell surface, cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Flow cytometry : 0.5-1ug/10^6 cells
Western blot : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml for 30 min at RT
Limitations This Connexin 32 antibody is available for research use only.
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Description

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

Application Notes

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Immunogen

Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Storage

Store the Connexin 32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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