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Home >> Antibodies >> Connexin 32 Antibody / GJB1

Connexin 32 Antibody / GJB1 [clone GJB1/1753] (V3304)

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  Catalog No Formulation Size Price (USD)  
Image V3304-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3304-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3304SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human liver with Connexin 32 antibody (clone GJB1/1753). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human liver with Connexin 32 antibody (clone GJB1/1753). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human tonsil with Connexin 32 antibody (clone GJB1/1753). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name GJB1/1753
Purity Protein G affinity chromatography
UniProt P08034
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.1-0.2ug/ml for 30 min at RT
Limitations This Connexin 32 antibody is available for research use only.
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Description

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32, also called Gap junction protein beta 1. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

Application Notes

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Immunogen

A recombinant human protein was used as the immunogen for the Connexin 32 antibody.

Storage

Store the Connexin 32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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