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Home >> Antibodies >> COMP Antibody / Cartilage Oligomeric Matrix Protein

COMP Antibody / Cartilage Oligomeric Matrix Protein (RQ4553)

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  Catalog No Formulation Size Price (USD)  
Image RQ4553 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HepG2 cells with COMP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HepG2 cells with COMP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human ovarian cancer with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human stomach cancer with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human esophagus squama cancer with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human intestinal cancer with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human cholangiocarcinoma cancer with COMP antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human placenta with COMP antibody at 0.5ug/ml. Expected molecular weight: 83-100 kDa depending on glycosylation level.
Flow cytometry testing of human HepG2 cells with COMP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COMP antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P49747
Localization Secreted
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This COMP is available for research use only.
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    Reactivity : Human

Description

COMP is a pentameric extracellular matrix protein that catalyzes the assembly of collagens and promotes formation of well-defined fibrils. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Application Notes

Optimal dilution of the COMP antibody should be determined by the researcher.

Immunogen

Amino acids Q505-A757 from the human protein were used as the immunogen for the COMP antibody.

Storage

After reconstitution, the COMP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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