Collagen 1 Antibody | R31258 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	R31258	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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Western blot testing of Collagen 1 antibody and Lane 1: rat lung; 2: human placenta; 3: rat testis tissue lysate. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).

IHC-P: Collagen 1 antibody testing of human intestine cancer tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with Collagen 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ICC testing of Collagen 1 antibody and NIH3T3 cells. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC-P: Collagen 1 antibody testing of rat lung tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human breast tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human tonsil tissue with Collagen 1 antibody (red) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE mouse lung tissue with Collagen 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC-P: Collagen 1 antibody testing of rat lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE rat lung tissue with Collagen 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous carcinoma tissue with Collagen 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Collagen 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human placenta, 2) rat skin, 3) mouse skin and 4) mouse NIH 3T3 cell lysate with Collagen 1 antibody. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P02452
Localization	Cytoplasmic
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunocytochemistry (FFPE) : 1-2ug/ml Immunofluorescence (FFPE) : 5ug/ml
Limitations	This Collagen 1 antibody is available for research use only.
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Description

Collagen type 1 alpha 1, also known as COL1A1, is a human gene that encodes the major component of type 1 collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type 1 collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Collagen type 1 is fibril-forming and found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Collagen 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen 1 antibody (100% rat homology).

Storage

After reconstitution, the Collagen 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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Collagen 1 Antibody (R31258)

Description

Application Notes

Immunogen

Storage

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