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Home >> Antibodies >> Collagen IV Antibody / COL4A2 (Canstatin fragment)

Collagen IV Antibody / COL4A2 (Canstatin fragment) (R30596)

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  Catalog No Formulation Size Price (USD)  
Image R30596 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC testing of frozen rat kidney tissue with Canstatin / Collagen IV antibody at 1ug/ml.
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IHC testing of frozen rat kidney tissue with Canstatin / Collagen IV antibody at 1ug/ml.
IHC testing of FFPE mouse lung tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of rat 1) kidney and 2) lung lysate with Canstatin / Collagen IV antibody at 0.5ug/ml. Expected molecular weight of Canstatin: ~24 kDa.
IHC testing of FFPE human breast cancer tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat kidney tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat kidney tissue with Canstatin / Collagen IV antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A431 cells with Canstatin / Collagen IV antibody (green) and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P08572
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml
Immunocytochemistry : 2-4ug/ml
Limitations This Collagen IV antibody is available for research use only.
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Description

Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, is one of the six subunits of Collagen IV and possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Application Notes

Titration of the Collagen IV antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Collagen IV COL4A2 (amino acids 1697-1712: LIRTHISRCQVCMKNL) was used as the immunogen for this Collagen IV antibody (100% homologous in human, mouse and rat). This amino acid sequence is from the Canstatin fragment of the protein.

Storage

The lyophilized Collagen IV antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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