Collagen I Antibody | COL1A1 | R32379 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	R32379	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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Immunofluorescent staining of FFPE human placental tissue with Collagen I antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE mouse lung tissue with Collagen I antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE rat lung tissue with Collagen I antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

IHC staining of FFPE human placental tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder urothelial carcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse lung tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat lung tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human placenta, 2) rat skin, 3) rat lung, 4) mouse skin and 5) mouse NIH 3T3 cell lysate with Collagen I antibody. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P02452
Localization	Cytoplasmic
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml
Limitations	This Collagen I antibody is available for research use only.
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Description

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Application Notes

Optimal dilution of the Collagen I antibody should be determined by the researcher.

Immunogen

Amino acids AGFDFSFLPQPPQEKAHDGGRYYRA were used as the immunogen for the Collagen I antibody.

Storage

After reconstitution, the Collagen I antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Collagen I Antibody / COL1A1 (R32379)

Description

Application Notes

Immunogen

Storage

Area of Research

Product Type

Protocols

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