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Home >> Antibodies >> COG4 Antibody / Conserved oligomeric Golgi complex subunit 4

COG4 Antibody / Conserved oligomeric Golgi complex subunit 4 (F54928)

  Catalog No Formulation Size Price (USD)  
Image F54928-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54928-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human K562 cell lysate with COG4 antibody. Predicted molecular weight ~89 kDa.
Western blot testing of human A2058 cell lysate with COG4 antibody. Predicted molecular weight ~89 kDa.
IHC testing of FFPE human skeletal muscle tissue with COG4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human Jurkat cells with COG4 antibody; Blue=isotype control, Green= COG4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q9H9E3
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This COG4 antibody is available for research use only.
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Description

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.

Application Notes

The stated application concentrations are suggested starting points. Titration of the COG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 757-785 from the human protein was used as the immunogen for the COG4 antibody.

Storage

Aliquot the COG4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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