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Home >> Antibodies >> CLOCK Antibody

CLOCK Antibody [clone ACCH-3] (RQ4730)

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Image RQ4730 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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IF/ICC staining of human HeLa cells with CLOCK antibody (green) and DAPI nuclear stain (blue).
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IF/ICC staining of human HeLa cells with CLOCK antibody (green) and DAPI nuclear stain (blue).
IHC staining of human thyroid carcinoma with CLOCK antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 293T cell lysate with CLOCK antibody. Predicted molecular weight ~95 kDa but routinely observed at 95-110 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name ACCH-3
Purity Affinity purified
UniProt O15516
Localization Cytoplasmic
Applications Western blot : 1:1000-1:5000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence/Immunocytochemistry : 1:50-1:200
Limitations This CLOCK antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse, Rat

Description

Circadian rhythms govern many key physiological processes that fluctuate with a period of approximately 24 hours. These processes include the sleep-wake cycle, glucose, lipid and drug metabolism, heart rate, hormone secretion, renal blood flow, and body temperature, as well as basic cellular processes such as DNA repair and the timing of the cell division cycle.

Application Notes

Optimal dilution of the CLOCK antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human CLOCK was used as the immunogen for the CLOCK antibody.

Storage

Store the CLOCK antibody at -20oC.

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