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Home >> Antibodies >> cKit Antibody

cKit Antibody (F40156)

  Catalog No Formulation Size Price (USD)  
Image F40156-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40156-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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cKit antibody used in western blot to detect KIT in serum-starved HeLa cell lysate (lane 1) and primate testis tissue lysate (2). Observed molecular weight: ~120/145kDa (precusor/mature).
cKit antibody was used in immunohistochemistry on breast carcinoma.
Flow cytometric analysis of HeLa cells using cKit antibody (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human, Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P10721
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This cKit antibody is available for research use only.
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Description

c-Kit is a Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. [UniProt]

Application Notes

Titration of the cKit antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 9-40 from the human protein was used as the immunogen for this cKit antibody.

Storage

Aliquot the cKit antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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