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Home >> Antibodies >> Cdk4 Antibody

Cdk4 Antibody (R30500)

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  Catalog No Formulation Size Price (USD)  
Image R30500 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with Cdk4 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with Cdk4 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human K562, 3) rat PC-12, 4) mouse NIH 3T3 and 5) mouse RAW264.7 cell lysate with Cdk4 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human MCF7 cells with Cdk4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cdk4 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P30285
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Cdk4 antibody is available for research use only.
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Description

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of mouse Cdk4 (RISAFRALQHSYLHKEESDAE) was used as the immunogen for this Cdk4 antibody.

Storage

After reconstitution, the Cdk4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

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