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Home >> Antibodies >> Cdk4 Antibody

Cdk4 Antibody (F52368)

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  Catalog No Formulation Size Price (USD)  
Image F52368-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52368-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of paraffin-embedded rat small intestine using Cdk4 antibody at 1:25.
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IHC testing of paraffin-embedded rat small intestine using Cdk4 antibody at 1:25.
IHC analysis of FFPE rat brain section using Cdk4 antibody at 1:25.
Fluorescent image of MCF-7 cells stained with Cdk4 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P35426
Localization Nuclear and cytoplasmic
Applications IHC (Paraffin) : 1:25
Immunofluorescence : 1:25
Limitations This Cdk4 antibody is available for research use only.
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Description

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Application Notes

Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 272-303 from the rat protein was used as the immunogen for this Cdk4 antibody.

Storage

Aliquot the Cdk4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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