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Home >> Antibodies >> CDH3 Antibody / P-Cadherin

CDH3 Antibody / P-Cadherin [clone 12H6] (V3329)

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  Catalog No Formulation Size Price (USD)  
Image V3329-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3329-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3329SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human A431 cell lysate with P-Cadherin / CDH3 antibody (clone 12H6). Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated).
Western blot testing of human A431 cell lysate with P-Cadherin / CDH3 antibody (clone 12H6). Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated).
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 12H6
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P22223
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Limitations This CDH3 antibody is available for research use only.
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Description

Recognizes a protein of 116kDa, identified as P-Cadherin, or Cadherin 3 or CDH3. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDH3 antibody to be titered up or down for optimal performance.

Immunogen

A human full length recombinant protein was used as the immunogen for this P-Cadherin / CDH3 antibody.

Storage

Store the CDH3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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