- Tel: 858.663.9055
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- Tel: 858.663.9055
- Email: info@nsjbio.com
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CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]
Optimal dilution of the CD99 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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