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Home >> Antibodies >> CD81 Antibody

CD81 Antibody [clone 1.3.3.22] (V3485)

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  Catalog No Formulation Size Price (USD)  
Image V3485-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3485-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3485SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human lymph node with CD81 antibody (clone 1.3.3.22). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using CD81 antibody (clone 1.3.3.22). Gray=unstained cells, Orange = CD81 antibody.
Flow cytometry staining of human MCF7 cells with CD81 antibody; Gray=unstained cells, Orange= CD81 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name 1.3.3.22
Purity Protein G affinity chromatography
UniProt P60033
Localization Cytoplasmic, membranous
Applications Functional studies : order BSA/sodium azide-free format
Flow cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Western blot : 0.5-1ug/ml for 2 hours at RT
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This CD81 antibody is available for research use only.
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Description

This mAb recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Application Notes

Optimal dilution of the CD81 antibody should be determined by the researcher.

Immunogen

Recombinant full length human protein was used as the immunogen for the CD81 antibody.

Storage

Store the CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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