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Home >> Antibodies >> CD79b Antibody

CD79b Antibody (R31586)

  Catalog No Formulation Size Price (USD)  
Image R31586 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of CD79b antibody and human cell lysate 1: Raji; 2: HL-60; 3: HUT; Predicted molecular weight: 26~39 kDa.
Western blot testing of CD79b antibody and recombinant human protein (0.5ng)
IHC-P: CD79b antibody testing of human tonsil tissue
IHC-P staining of mouse spleen tissue
IHC-P staining of rat spleen tissue
Immunofluorescent staining of FFPE human A431 cells with CD79b antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with CD79b antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 974
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Limitations This CD79b antibody is available for research use only.
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Description

Cluster of Differentiation 79b, is a human gene mapped to 17q23.3. The CD79b protein together with the relatedCD79aprotein, forms a dimer associated with membrane bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). CD79b also enhances phosphorylation of CD79a, possibly by recruiting kinases, or by recruiting proteins which bind to CD79a and protect it from dephosphorylation.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the CD79b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 29-229) was used as the immunogen for this CD79b antibody.

Storage

After reconstitution, the CD79b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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