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Home >> Antibodies >> CD68 Antibody

CD68 Antibody [clone CDLA68-1] (V7045)

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  Catalog No Formulation Size Price (USD)  
Image V7045-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7045-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7045SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7045IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Flow cytometry testing of human U-87 MG cells with CD68 antibody (clone CDLA68-1); Red=isotype control, Blue= CD68 antibody.
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Flow cytometry testing of human U-87 MG cells with CD68 antibody (clone CDLA68-1); Red=isotype control, Blue= CD68 antibody.
IHC testing of human tonsil and CD68 antibody (CDLA68-1). HIER: staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of human histiocytoma tissue and CD68 antibody (CDLA68-1). HIER: staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CDLA68-1
Purity Protein G affinity chromatography
UniProt P34810
Localization Cytoplasmic, membrane
Applications Flow cytometry : 0.5-1ug/million cells in 0.1ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This CD68 antibody is available for research use only.
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Description

CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]

Application Notes

Optimal dilution of the CD68 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the CD68 antibody.

Storage

Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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