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Home >> Antibodies >> CD6 Antibody

CD6 Antibody [clone SPV-L14] (V2961)

  Catalog No Formulation Size Price (USD)  
Image V2961-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2961-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2961SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human MOLT-4 cells with CD6 antibody (green, clone SPV-L14) and Reddot nuclear stain (red).
Flow cytometry testing of human MOLT-4 cells with CD6 antibody (clone SPV-L14); Red=isotype control, Blue= CD6 antibody.
SDS-PAGE analysis of purified, BSA-free CD6 antibody (clone SPV-L14) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPV-L14
Purity Protein G affinity chromatography
UniProt P30203
Localization Cell surface and cytoplasmic
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD6 antibody is available for research use only.
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Description

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Application Notes

Optimal dilution of the CD6 antibody should be determined by the researcher.

Immunogen

A CD8+ cytotoxic T-cell clone was used as the immunogen for the CD6 antibody.

Storage

Store the CD6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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