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Home >> Antibodies >> CD57 Antibody / HNK-1

CD57 Antibody / HNK-1 [clone NK1/7566] (V4219)

  Catalog No Formulation Size Price (USD)  
Image V4219-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4219-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4219SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human Daudi cell lysate with CD57 antibody (clone NK1/7566). Predicted molecular weight ~38 kDa.
SDS-PAGE analysis of purified, BSA-free CD57 antibody (clone NK1/7566) as confirmation of integrity and purity.
IHC staining of FFPE human lymph node tissue with CD57 antibody (clone NK1/7566). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, lambda
Clone Name NK1/7566
Purity Protein A/G affinity
UniProt Q9P2W7
Localization Cell surface, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD57 antibody is available for research use only.
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Description

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Application Notes

Optimal dilution of the CD57 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD57 antibody.

Storage

Aliquot the CD57 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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