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Home >> Antibodies >> CD56 Antibody

CD56 Antibody [clone NCAM/7524] (V4191)

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  Catalog No Formulation Size Price (USD)  
Image V4191-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4191-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4191SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human cerebellum tissue with CD56 antibody (clone NCAM/7524). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human cerebellum tissue with CD56 antibody (clone NCAM/7524). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD56 antibody (clone NCAM/7524) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NCAM/7524
Purity Protein A/G affinity
UniProt P13591 , P13592
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD56 antibody is available for research use only.
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Description

This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Application Notes

Optimal dilution of the CD56 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein (within amino acids 400-650) from the human protein was used as the immunogen for the CD56 antibody.

Storage

Aliquot the CD56 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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