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Home >> Antibodies >> CD53 Antibody

CD53 Antibody [clone 63-5A3] (V3018)

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  Catalog No Formulation Size Price (USD)  
Image V3018-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3018-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3018SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free CD53 Antibody (clone 63-5A3). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free CD53 Antibody (clone 63-5A3). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name 63-5A3
Purity Protein G affinity chromatography
UniProt P19397
Localization Cell surface
Applications Functional studies (order BSA/sodium azide-free format) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD53 antibody is available for research use only.
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  • Applications : Functional studies, FACS, IF
    Reactivity : Human
    Citation  Citations(2)

Description

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

Application Notes

Optimal dilution of the CD53 antibody should be determined by the researcher.

Immunogen

Human Sezary cells were used as the immunogen for the CD53 antibody.

Storage

Store the CD53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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