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Home >> Antibodies >> CD53 Antibody

CD53 Antibody [clone 161-2] (V3019)

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  Catalog No Formulation Size Price (USD)  
Image V3019-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3019-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3019SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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SDS-PAGE Analysis of Purified, BSA-Free CD53 Antibody (clone 161-2). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free CD53 Antibody (clone 161-2). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name 161-2
Purity Protein G affinity chromatography
UniProt P19397
Localization Cell surface
Applications Functional studies (order BSA/sodium azide-free format) :
Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD53 antibody is available for research use only.
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  • Applications : Functional studies, FACS, IF
    Reactivity : Human
    Citation  Citations(4)

Description

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This mAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Application Notes

Optimal dilution of the CD53 antibody should be determined by the researcher.

Immunogen

Stimulated human leukocytes were used as the immunogen for the CD53 antibody.

Storage

Store the CD53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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