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Home >> Antibodies >> CD47 Antibody / Integrin Associated Protein / IAP

CD47 Antibody / Integrin Associated Protein / IAP (RQ4922)

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  Catalog No Formulation Size Price (USD)  
Image RQ4922 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human PBM cells with CD47 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD47 antibody.
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Flow cytometry testing of human PBM cells with CD47 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD47 antibody.
IHC staining of FFPE human prostate cancer tissue with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
Western blot testing of human placenta lysate with CD47 antibody at 0.5ug/ml. Predicted molecular weight: 35~60 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q08722
Localization Cell surface, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CD47 antibody is available for research use only.
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Description

CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Application Notes

Optimal dilution of the CD47 antibody should be determined by the researcher.

Immunogen

Amino acids Q19-E323 from the human protein were used as the immunogen for the CD47 antibody.

Storage

After reconstitution, the CD47 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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