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Home >> Antibodies >> CD44 Antibody

CD44 Antibody [clone AGI-3] (RQ5462)

  Catalog No Formulation Size Price (USD)  
Image RQ5462 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ul 429
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IHC staining of FFPE human skin with CD44 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human TF-1 cell lysate with CD44 antibody. Predicted molecular weight ~81 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AGI-3
Purity Affinity purified
UniProt P16070
Localization Cell surface, cytoplasmic
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This CD44 antibody is available for research use only.
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Description

The protein encoded by the CD44 gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [RefSeq]

Application Notes

Optimal dilution of the CD44 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human CD44 was used as the immunogen for the CD44 antibody.

Storage

Store the CD44 antibody at -20oC.

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