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Home >> Antibodies >> CD40L Antibody / TRAP / CD154

CD40L Antibody / TRAP / CD154 [clone CD40LG/2763] (V8248)

  Catalog No Formulation Size Price (USD)  
Image V8248-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8248-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8248SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Flow testing of human Jurkat cells with CD40L antibody (blue) and isotype control (red).
Immunofluorescent staining of human Jurkat cells with CD40L antibody (green) and Reddot nuclear stain (red).
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human spleen with CD40L antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD40L antibody (clone CD40LG/2763). These results demonstrate the foremost specificity of the CD40LG/2763 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CD40L antibody (clone CD40LG/2763) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CD40LG/2763
Purity Protein G affinity chromatography
UniProt P29965
Localization Cell surface, secreted
Applications ELISA (order BSA-free format for coating) :
Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD40L antibody is available for research use only.
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Description

CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

Application Notes

Optimal dilution of the CD40L antibody should be determined by the researcher.

Immunogen

A portion of amino acids 108-261 from the human protein was used as the immunogen for the CD40L antibody.

Storage

Store the CD40L antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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