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Home >> Antibodies >> CD3e Antibody

CD3e Antibody [clone UCHT1] (V3760)

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  Catalog No Formulation Size Price (USD)  
Image V3760-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3760-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3760SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human Jurkat cells with CD3e antibody (clone UCHT1, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of human Jurkat cells with CD3e antibody (clone UCHT1, green) and Reddot nuclear stain (red).
Flow cytometry testing of human Jurkat cells with CD3e antibody (clone UCHT1); Red=isotype control, Blue= CD3e antibody.
SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone UCHT1) as confirmation of integrity and purity.
Flow cytometry staining of lymphocyte-gated human PBM cells with CF640R-labeled CD3e antibody (clone UCHT1). Gray=unstained, Red=CF640R-CD3e antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name UCHT1
Purity Protein G affinity chromatography
UniProt P07766
Localization Cell surface and cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (Acetone-fixed frozen tissues) : 1-2ug/ml for 30 min at RT
Limitations This CD3e antibody is available for research use only.
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Description

This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Application Notes

Optimal dilution of the CD3e antibody should be determined by the researcher.

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor were used as the immunogen for the CD3e antibody.

Storage

Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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