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Home >> Antibodies >> CD35 Antibody / CR1

CD35 Antibody / CR1 [clone To5] (V7691)

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  Catalog No Formulation Size Price (USD)  
Image V7691-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7691-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7691SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil with CD35 antibody (clone To5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with CD35 antibody (clone To5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD35 antibody (clone To5) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name To5
Purity Protein G affinity chromatography
UniProt P17927
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD35 antibody is available for research use only.
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Description

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Application Notes

Optimal dilution of the CD35 antibody should be determined by the researcher.

Immunogen

Follicular dendritic cells (FDC) from the tonsil were used as the immunogen for the CD35 antibody.

Storage

Store the CD35 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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