CD34 Antibody ICO-115 PE Conjugate | V2065PE NSJ Bio NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2065PE-100T	500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 Tests	519
Formulation

Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (ICO-115, red) and isotype control antibody (green). The PPI-negative cell population was gated for analysis.

Availability	1-3 business days
Species Reactivity	Human and Rat. Other species not known.
Format	PE Conjugate
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	ICO-115
Purity	Protein G affinity chromatography
Gene ID	947 (Human)
Localization	Cell surface
Applications	Flow Cytometry : 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood) Immunofluorescence : 1:50-1:100
Limitations	This antibody is available for research use only.
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Description

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.