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  • Email: info@nsjbio.com
Home >> Antibodies >> CD28 Antibody

CD28 Antibody [clone CB28] (V2051)

  Catalog No Formulation Size Price (USD)  
V2051-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2051-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2051SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CB28
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 940
Localization Cell surface
Applications Functional Testing : order BSA/azide-free format
Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Limitations This CD28 antibody is available for research use only.

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Description

This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical Tcell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing Tcells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. it is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD28 antibody to be titered up or down for optimal performance.

Immunogen

Recombinant human protein was used as the immunogen for this CD28 antibody.

Storage

Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Tp44, CD28 antibody

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