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  • Email: info@nsjbio.com
Home >> Antibodies >> CD28 Antibody

CD28 Antibody [clone C28/77] (V2054)

  Catalog No Formulation Size Price (USD)  
V2054-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2054-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2054SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C28/77
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 940
Localization Cell surface
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Limitations This CD28 antibody is available for research use only.

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Description

This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Recombinant human CD28 protein was used as the immunogen for this antibody.

Storage

Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

T-cell-specific surface glycoprotein CD28; Tp44, CD28 antibody

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