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Home >> Antibodies >> CD1a Antibody

CD1a Antibody [clone O10] (V2025)

  Catalog No Formulation Size Price (USD)  
V2025-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2025-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2025SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2025IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human skin stained with CD1a antibody (O10).
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name O10
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 909
Localization Cell surface, cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This CD1a antibody is available for research use only.

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Description

At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict Tcell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. CD1a antibody labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibody against TTF-1 and CD5, CD1a antibody is useful in distinguishing between pulmonary and thymic neoplasms since it is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. CD1a antibody is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD1a antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human thymus cells were used as the immunogen for this CD1a antibody.

Storage

Store the CD1a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Epidermal dendritic cell marker, T cell surface antigen T6 / Leu 6

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