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Home >> Antibodies >> CD19 Antibody

CD19 Antibody (F44318)

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  Catalog No Formulation Size Price (USD)  
Image F44318-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F44318-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.
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Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.
CD19 antibody western blot analysis in Raji lysate. Expected size is 60~100 kDa depending on glycosylation level.
Western blot analysis of CD19 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (2). Expected size is 60~100 kDa depending on glycosylation level.
Immunofluorescence analysis of CD19 antibody with paraffin-embedded human lymph tissue. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
Flow cytometric analysis of CEM cells using CD19 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human lymph stained with CD19 antibody
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P15391
Localization Cell surface, cytoplasmic
Applications Western blot : 1:500-100
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
IHC (Paraffin) : 1:10-1:50
Limitations This CD19 antibody is available for research use only.
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Description

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Application Notes

Titration of the CD19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 143-172 from the human protein was used as the immunogen for this CD19 antibody.

Storage

Aliquot the CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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