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Home >> Antibodies >> CD185 Antibody / CXCR5 / BLR1 / MDR15

CD185 Antibody / CXCR5 / BLR1 / MDR15 [clone CXCR5/8279R] (V4214)

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  Catalog No Formulation Size Price (USD)  
Image V4214-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4214-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4214SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with CD185 antibody (clone CXCR5/8279R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with CD185 antibody (clone CXCR5/8279R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD185 antibody (clone CXCR5/8279R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CXCR5/8279R
Purity Protein A/G affinity
UniProt P32302
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD185 antibody is available for research use only.
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Description

Burkitt s lymphoma receptor 1 (Blr1), also called CD185, CXCR5 and MDR15, is a lymphocyte specific chemokine receptor expressed at low levels in secondary lymphoid tissues and in defined structures of the cerebellum. The G protein-coupled receptor has significant homology to other chemokine receptors. Stimulation of Blr1 by its ligand, B-lymphocyte chemoattractant (BLC) results in an influx of calcium into the cell and the chemotaxis of the cell. Blr1 is required for B-cell migration into splenic and Peyer s patch follicles. BLC expression in Peyer s patches is highest in germinal centers, where B cells undergo somatic mutation and affinity maturation.

Application Notes

Optimal dilution of the CD185 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD185 antibody.

Storage

Aliquot the CD185 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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