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Home >> Antibodies >> CD16 Antibody

CD16 Antibody [clone C16/1045] (V8858)

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  Catalog No Formulation Size Price (USD)  
Image V8858-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8858-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8858SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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FACS staining of lymphocyte-gated human PBM cells with CD16 antibody (violet, clone C16/1045), and unstained cells (gray).
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FACS staining of lymphocyte-gated human PBM cells with CD16 antibody (violet, clone C16/1045), and unstained cells (gray).
SDS-PAGE analysis of purified, BSA-free CD16 antibody (clone C16/1045) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C16/1045
Purity Protein A/G affinity
UniProt P08637
Localization Cell Surface
Applications Flow cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Limitations This CD16 antibody is available for research use only.
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Description

It recognizes CD16 (Fc gamma RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80kDa. Two similar genes represent CD16, CD16A (Fc gamma RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gamma RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Application Notes

Optimal dilution of the CD16 antibody should be determined by the researcher.

Immunogen

Recombinant human CD16 protein was used as the immunogen for the CD16 antibody.

Storage

Aliquot the CD16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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