CD147 Antibody / Basigin (R32714)

Western blot testing of human 1) HeLa, 2) placenta, 3) Jurkat, 4) U-2 OS, 5) K562, 6) MCF-7, 7) COLO-320, 8) HepG2 and 9) PANC-1 lysate with CD147 antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.

IHC testing of FFPE human tonsil tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human intestinal cancer tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human lung cancer tissue with CD147 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry testing of human HeLa cells with CD147 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD147 antibody.

Flow cytometry testing of human A549 cells with CD147 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD147 antibody.

Immunofluorescent staining of FFPE human intestinal cancer with CD147 antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IF/ICC staining of FFPE human A549 cells with CD147 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

IF/ICC staining of FFPE human HeLa cells with CD147 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with CD147 antibody. Expected molecular weight: 27-66 kDa depending on level of glycosylation.