- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD147 Antibody [clone 8D6] (V2015)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V2015-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V2015-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V2015SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 | |
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V2015IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 519 |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 8D6 |
| Purity | Protein G purified CD147 antibody |
| Buffer | 1X PBS, pH 7.4 |
| Gene ID | 682 |
| Localization | Cell surface, cytoplasmic |
| Applications | Inhibits homotypic aggregation : adhesion to matrix proteins : and migation through matrigel (order BSA/sodium azide-free format) : Flow cytometry : 0.5-1ug/10^6 cells Immunofluorescence : 1:100-1:200 Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT |
| Limitations | This CD147 antibody is available for research use only. |
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This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.
Store the CD147 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor, CD147 antibody
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