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Home >> Antibodies >> CD11b Antibody / MAC-1 / ITGAM

CD11b Antibody / MAC-1 / ITGAM (RQ4351)

  Catalog No Formulation Size Price (USD)  
Image RQ4351 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen and 4) mouse thymus lysate with CD11b antibody at 0.5ug/ml. Expected molecular weight: ~95 kDa and ~170 kDa.
Flow cytometry testing of human HL-60 cells with CD11b antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD11b antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P11215
Localization Cytoplasmic, membranous
Applications Western blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CD11b antibody is available for research use only.
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Description

CD11b, or Macrophage-1 alpha (MAC-1), is a glycoprotein encoded by the ITGAM gene. It has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the CD11b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A recombinant human protein corresponding to amino acids N129-E336 was used as the immunogen for the CD11b antibody.

Storage

After reconstitution, the CD11b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Granulocyte marker

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