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Home >> Antibodies >> C-terminal-binding protein 2 Antibody / CTBP2

C-terminal-binding protein 2 Antibody / CTBP2 [clone 7F3E1] (RQ7571)

  Catalog No Formulation Size Price (USD)  
Image RQ7571 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse colon tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse rat tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid cancer tissue with C-terminal-binding protein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human SiHa, 3) human COLO-320, 4) human SW620, 5) rat stomach, 6) rat brain, 7) mouse stomach and 8) mouse brain tissue lysate with C-terminal-binding protein 2 antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human U-87 MG cells with C-terminal-binding protein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= C-terminal-binding protein 2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 7F3E1
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P56545
Localization Nucleus, Cell junction
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This C-terminal-binding protein 2 antibody is available for research use only.
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Description

The E1a region of group C adenoviruses encodes 2 nearly identical proteins that are largely responsible for the oncogenic properties of adenoviruses. The CTBP1 protein binds to the C-terminal half of these E1A proteins. It's predicted that CTBP2 is a 445-amino acid protein and it is 72% identical to CTBP1. The CTBP2 gene is mapped to chromosome 10q26.13. CTBP2 is a mammalian corepressor that targets diverse transcriptional regulators. It bounds the short medial portion of delta-EF1 containing the PLDLSL motif and it enhances transrepression activity of delta-EF1.

Application Notes

Optimal dilution of the C-terminal-binding protein 2 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H321-Q445) was used as the immunogen for the C-terminal-binding protein 2 antibody.

Storage

After reconstitution, the C-terminal-binding protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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