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Home >> Antibodies >> BrdU Antibody / Bromodeoxyuridine

BrdU Antibody / Bromodeoxyuridine [clone BRD2888R] (V3928)

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  Catalog No Formulation Size Price (USD)  
Image V3928-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3928-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3928SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of BrdU-incorporated FFPE mouse kidney with BrdU antibody (clone BRD2888R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of BrdU-incorporated FFPE mouse kidney with BrdU antibody (clone BRD2888R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free BrdU antibody (clone BRD2888R) as confirmation of integrity and purity.
Species Reactivity All species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name BRD2888R
Purity Protein A affinity chromatography
Buffer 1X PBS, pH 7.4
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This BrdU antibody is available for research use only.
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Description

This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.

Immunogen

BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.

Storage

Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Bromodeoxyuridine, BUdr, BrdU antibody

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   2.   View all recombinant antibodies

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