BMI1 Antibody [clone BMI1/2823] (V8080)

Western blot testing of mouse NIH3T3 cell lysate with BMI1 antibody. Predicted molecular weight: 37-43 kDa.

IHC staining of FFPE human colon carcinoma with BMI1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with BMI1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma with BMI1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells with BMI1 antibody (green) and Phalloidin (red).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using BMI1 antibody (clone BMI1/2823). These results demonstrate the foremost specificity of the BMI1/2823 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free BMI1 antibody as confirmation of integrity and purity.