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Home >> Antibodies >> BLIMP1 Antibody / PRDM1

BLIMP1 Antibody / PRDM1 (RQ4227)

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  Catalog No Formulation Size Price (USD)  
Image RQ4227 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) A549, 3) 293T, 4) MCF7 and 5) 22RV1 cell lysate with PRDM1 / BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
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Western blot testing of human 1) HeLa, 2) A549, 3) 293T, 4) MCF7 and 5) 22RV1 cell lysate with PRDM1 / BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
Western blot testing of rat 1) thymus, 2) spleen, 3) stomach, 4) lung and mouse 5) thymus, 6) spleen, 7) stomach, 8) lung and 9) HEPA1-6 lysate with BLIMP1 antibody at 0.5ug/ml. Predicted molecular weight: ~92 kDa.
IHC testing of FFPE human breast cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human placental tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with BLIMP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
UniProt O75626
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This BLIMP1 antibody is available for research use only.
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Description

PR domain zinc finger protein 1, also known as B Lymphocyte Induced Maturation Protein 1 (BLIMP-1), is a protein that in humans is encoded by the PRDM1 gene. This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.

Application Notes

Optimal dilution of the BLIMP1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids M1-Q230 was used as the immunogen for the PRDM1 / BLIMP1 antibody.

Storage

After reconstitution, the BLIMP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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