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Home >> Antibodies >> beta-Catenin Antibody

beta-Catenin Antibody (V2103)

  Catalog No Formulation Size Price (USD)  
V2103-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2103-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2103SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2103IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
Formalin/paraffin breast ductal carcinoma stained with beta-Catenin antibody. Note membrane staining in ductal carcinoma.
Formalin/paraffin breast lobular carcinoma stained with beta-Catenin antibody. Note cytoplasmic staining in lobular carcinoma.
Western blot testing of human and mouse samples using beta-Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
Species Reactivity Human and Mouse. Other species not known.
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 1499
Localization Cell surface and cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Limitations This beta-Catenin antibody is available for research use only.

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Description

Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, it is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution throughout the cytoplasm results in a diffuse cytoplasmic pattern. Staining with beta-catenin antibody and E-cadherin antibody helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A synthetic peptide from the middle of beta-Catenin / p120 protein was used as the immunogen.

Storage

Store the beta-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1, p120 antibody, beta-Catenin antibody

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